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700 800 900 Surface Temperature (deg C)

The Tracing Image cannot be selected and moved the same way as other objects on your page. Instead, you must move the Tracing Image using menu commands. You have several options for adjusting the Tracing Image s position to better fit your design. First, you can align the Tracing Image with any object on your page by selecting the object and then choosing View Tracing Image Align with Selection. This lines up the upper-left corner of the Tracing Image with the upper-left corner of the bounding box of the object you ve selected. To precisely or visually move the Tracing Image to a specific location, choose View Tracing Image Adjust Position. Enter the x and y coordinates into their respective boxes in the Adjust Tracing Image Position dialog box, as shown in Figure 11-22. For more hands-on positioning, use the arrow keys to nudge the tracing AP element up, down, left, or right, one pixel at a time. Holding down the Shift key while pressing the arrow keys moves the Tracing Image in 5-pixel increments. Finally, you can return the Tracing Image to its default location of 9 pixels down from the top and 11 pixels in from the left by choosing View Tracing Image Reset Position. FIGURE 11-22

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The pressure drop for gas ow only in the empty pipe is then determined using eq. (7-17): Pgas = 4f V2 L 2 D 4(0.00259)(4.97)(21.57)2 (0.924) = = 71.87 Pa or 0.00072 bar 2 0.154

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$TheT - emits a virtual W - , converting to a v,. The W - decays to all energetically allowed fermion-antifermion pairs with equal probability (weak universality-see Exercise 9.4): ( e v e ) , ( p u p ) , (ad), where phase-space factors, Cabibbo mixing, and QCD effects on the quarks are ignored. Thus (10.6) is f if the quarks are uncolored, and $ if there are three colors of u and d.

11.3.2.2. Immunoblotting. Connexin-43 and keratins 3 and 12 are detected by immunoblotting of proteins from cell lysates with procedures described in Section 11.4. ABCG2 can be immune precipitated from cell lysates after cell surface biotinylation as described below. Antibodies used are the same as previously described. 11.3.3. Cryopreservation Brie y, for LSC cells, trypsinize, count, pellet by centrifugation, and resuspend the cells in freezing medium at 2 5 106 cells/mL with 1 mL per vial. Chill at a controlled rate of 1 C/h, using a commercial freezing box lled with isopropanol in a 80 C freezer overnight. On the next day, transfer the vials to liquid nitrogen. Freezing medium: 70% culture medium (DMEM/F-12), 20% FBS, 10% DMSO. Make fresh each time before freezing cells. 11.3.4. Variations and Applications of the Method LSC can be cultured as limbal explants or puri ed by FACS for side population cells. 11.3.4.1. Explant Culture. After Dispase II digestion of human cornea (see Protocol 11.3), isolate the limbal epithelium and cut it into pieces about 1 mm in diameter. Seed these pieces on 3T3 feeder layers (see Protocol 11.2) or on amniotic membrane (see Protocol 11.1) with the epithelial side up. The cells will migrate out from the tissue over a period of 2 4 weeks.

The Find/Change dialog box lets you search for object attributes, not just text attributes. 11 covers object search and replace. n

Operator = < > or @@ (Mac); < > (PC) > What It Means Means equal. The test is true if the items on both sides of the sign are exactly equal in value. For example, the statement 1 + 1 = 2 is true. Means not equal to. The test is true if the items on both sides of the sign are not equal in value. For example, Apple < > Banana is true. Means greater than. The test is true if the value of the item at the left of the sign exceeds the value of item to the right. For example, 3 > 2 is true. For text arguments, greater than means later in the standard collating sequence (for example, alphabetic order).